验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66379 targets Beta Catenin in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, pig |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | catenin (cadherin-associated protein), beta 1, 88kDa |
别名 | b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286, β-Catenin |
计算分子量 | 781 aa, 86 kDa |
观测分子量 | 92 kDa |
GenBank蛋白编号 | NM_001904 |
基因名称 | Beta Catenin |
Gene ID (NCBI) | 1499 |
ENSEMBL Gene ID | ENSG00000168036 |
RRID | AB_2923919 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P35222 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Beta Catenin antibody CL594-66379 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |