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验证数据展示

  • IF Staining of MCF-7 using CL488-66379

    IF Staining of MCF-7 using CL488-66379

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Beta Catenin antibody (CL488-66379, Clone: 1B8A1 ) at dilution of 1:200, Alpha Tubulin antibody (11224-1-AP, red).

  • FC experiment of MCF-7 using CL488-66379

    FC experiment of MCF-7 using CL488-66379

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Catenin (CL488-66379, Clone:1B8A1) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO验证

CoraLite® Plus 488-conjugated Beta Catenin Monoclonal antibody

Beta Catenin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66379

产品说明书

CloneNo. 1B8A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66379-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66379 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat
计算分子量 781 aa, 86 kDa
GenBank蛋白编号NM_001904
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2883323
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP35222
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Beta Catenin antibody CL488-66379Download protocol
Standard Protocols
Click here to view our Standard Protocols