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CoraLite® Plus 488-conjugated C1qC Monoclonal antibody

C1qC Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5B4

Cat no : CL488-66268

Print datasheet

Synonyms

C1Q C, C1QC, C1QG, FLJ27103

验证数据展示

  • IF Staining of HUVEC using CL488-66268

    IF Staining of HUVEC using CL488-66268

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 C1qC antibody (CL488-66268, Clone: 1C5B4 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHUVEC cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy C1QC Ready-To-Use IHC Kit. C1QC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66268-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66268 targets C1qC in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen C1qC fusion protein Ag10233 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name complement component 1, q subcomponent, C chain
Synonyms C1Q C, C1QC, C1QG, FLJ27103
Calculated Molecular Weight 245 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC009016
Gene Symbol C1QC
Gene ID (NCBI) 714
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP02747
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C1qC antibody CL488-66268Download protocol
Standard Protocols
Click here to view our Standard Protocols