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验证数据展示

  • IF Staining of HUVEC using CL488-66268

    IF Staining of HUVEC using CL488-66268

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 C1qC antibody (CL488-66268, Clone: 1C5B4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated C1qC Monoclonal antibody

C1qC Monoclonal Antibody for IF/ICC
Cat No. CL488-66268

产品说明书

CloneNo. 1C5B4

宿主/亚型

Mouse / IgM

种属反应性

human

应用

IF/ICC

别名

C1Q C, C1QC, C1QG, FLJ27103

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHUVEC cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy C1QC Ready-To-Use IHC Kit. C1QC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66268-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66268 targets C1qC in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 C1qC fusion protein Ag10233 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 1, q subcomponent, C chain
别名 C1Q C, C1QC, C1QG, FLJ27103
计算分子量 245 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC009016
基因名称 C1QC
Gene ID (NCBI) 714
RRIDAB_2934453
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDP02747
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C1qC antibody CL488-66268Download protocol
Standard Protocols
Click here to view our Standard Protocols