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CoraLite® Plus 488-conjugated C1qC Monoclonal antibody

C1qC Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5B4

Cat no : CL488-66268

Print datasheet

Synonyms

C1Q C, C1QC, C1QG, FLJ27103

验证数据展示

  • IF Staining of HUVEC using CL488-66268

    IF Staining of HUVEC using CL488-66268

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 C1qC antibody (CL488-66268, Clone: 1C5B4 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHUVEC cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy C1QC Ready-To-Use IHC Kit. C1QC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66268-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66268 targets C1qC in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen C1qC fusion protein Ag10233 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name complement component 1, q subcomponent, C chain
Synonyms C1Q C, C1QC, C1QG, FLJ27103
Calculated Molecular Weight 245 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC009016
Gene Symbol C1QC
Gene ID (NCBI) 714
RRIDAB_2934453
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP02747
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C1qC antibody CL488-66268Download protocol
Standard Protocols
Click here to view our Standard Protocols