• Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated C5aR Polyclonal antibody

C5aR Polyclonal Antibody for IF/ICC
Cat No. CL488-21316

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

C5A, C5a R, C5AR, C5AR1, C5R1, CD88

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C5AR1 Ready-To-Use IHC Kit. C5AR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21316-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21316 targets C5aR in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 C5aR fusion protein Ag15968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 5a receptor 1
别名 C5A, C5a R, C5AR, C5AR1, C5R1, CD88
计算分子量 350 aa, 39 kDa
观测分子量 45 kDa
GenBank蛋白编号BC008982
基因名称 C5aR
Gene ID (NCBI) 728
RRIDAB_3084051
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21730
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C5aR antibody CL488-21316Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...