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验证数据展示

  • IF Staining of HepG2 using CL488-21316

    IF Staining of HepG2 using CL488-21316

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 C5aR antibody (CL488-21316) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated C5aR Polyclonal antibody

C5aR Polyclonal Antibody for IF/ICC
Cat No. CL488-21316

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

C5A, C5a R, C5AR, C5AR1, C5R1, CD88

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C5AR1 Ready-To-Use IHC Kit. C5AR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21316-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21316 targets C5aR in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen C5aR fusion protein Ag15968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 5a receptor 1
Synonyms C5A, C5a R, C5AR, C5AR1, C5R1, CD88
Calculated Molecular Weight 350 aa, 39 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008982
Gene Symbol C5aR
Gene ID (NCBI) 728
RRIDAB_3084051
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21730
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C5aR antibody CL488-21316Download protocol
Standard Protocols
Click here to view our Standard Protocols