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C9orf72 Polyclonal antibody
C9orf72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, transfected cells, HeLa cells, Sp2/0 cells, SH-SY5Y cells, rat brain tissue, mouse testis tissue, HEK-293 cells, NIH/3T3 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human gliomas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22637-1-AP targets C9orf72 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, caenorhabditis elegans |
| Immunogen | C9orf72 fusion protein Ag18326 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromosome 9 open reading frame 72 |
| Synonyms | Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT |
| Calculated Molecular Weight | 481 aa, 54 kDa |
| Observed Molecular Weight | 50-54 kDa |
| GenBank Accession Number | BC020851 |
| Gene Symbol | C9orf72 |
| Gene ID (NCBI) | 203228 |
| RRID | AB_10953528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96LT7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf72 antibody 22637-1-AP | Download protocol |
| IHC protocol for C9orf72 antibody 22637-1-AP | Download protocol |
| IF protocol for C9orf72 antibody 22637-1-AP | Download protocol |
| IP protocol for C9orf72 antibody 22637-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science C9orf72 is required for proper macrophage and microglial function in mice.
| ||
Nat Med Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. | ||
Cell Metab C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.
| ||
Nat Neurosci Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.
| ||
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy
| ||
Sci Transl Med Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.
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