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验证数据展示

  • IF Staining of mouse testis using CL594-22637

    IF Staining of mouse testis using CL594-22637

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 C9orf72 antibody (CL594-22637) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-22637

    IF Staining of mouse testis using CL594-22637

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 C9orf72 antibody (CL594-22637) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated C9orf72 Polyclonal antibody

C9orf72 Polyclonal Antibody for IF-P
Cat No. CL594-22637

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

C9orf72, RP11 27J8.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22637-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-22637 targets C9orf72 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen C9orf72 fusion protein Ag18326 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 9 open reading frame 72
Synonyms C9orf72, RP11 27J8.2
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight 50-54 kDa, 25-30 kDa, 43 kDa, 68-72 kDa
GenBank Accession NumberBC020851
Gene Symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_2934707
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LT7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)

实验方案

Product Specific Protocols
IF protocol for CL594 C9orf72 antibody CL594-22637Download protocol
Standard Protocols
Click here to view our Standard Protocols