验证数据展示

WB analysis using 10427-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 10427-2-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was stained with CL594-Phalloidin (red).
WB analysis using 10427-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 10427-2-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calnexin (10427-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of HEK-293 using 10427-2-AP
WB result of Calnexin antibody (10427-2-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells.
IHC staining of human breast cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Calnexin antibody included
IHC staining of human breast cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Calnexin antibody included
IHC staining of human colon using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10427-2-AP (Calnexin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Calnexin antibody included
IF Staining of HepG2 using 10427-2-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

Calnexin Polyclonal antibody

Calnexin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10427-2-AP

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Publications(447)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Synonyms

CANX, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90, CNX

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (447)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, RAW 264.7 cells
Positive IHC detected in	human breast cancer tissue, human cervical cancer tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, SKOV-3 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 387 publications below
IHC	See 3 publications below
IF	See 64 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

产品信息

10427-2-AP targets Calnexin in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited Applications	WB, IHC, IF, CoIP, ELISA
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, hamster, goat
Immunogen	Calnexin fusion protein Ag0697 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	calnexin
Synonyms	CANX, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90, CNX
Calculated Molecular Weight	90 kDa
Observed Molecular Weight	90 kDa
GenBank Accession Number	BC003552
Gene Symbol	Calnexin
Gene ID (NCBI)	821
RRID	AB_2069033
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27824
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

实验方案

Product Specific Protocols
WB protocol for Calnexin antibody 10427-2-AP	Download protocol
IHC protocol for Calnexin antibody 10427-2-AP	Download protocol
IF protocol for Calnexin antibody 10427-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Nature A virally encoded high-resolution screen of cytomegalovirus dependencies Authors - Yaara Finkel View Article
human	WB	Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression Authors - Shiqiao Qiu View Article
mouse	WB	Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies Authors - Liang Guo View Article
human	WB	Mol Cancer Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity Authors - Can Can View Article
human	WB	Cell Discov A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma Authors - Xifei Jiang View Article
		Immunity The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity Authors - Tian Chen View Article

验证数据展示

WB analysis using 10427-2-AP

IF Staining of HepG2 using 10427-2-AP

WB analysis using 10427-2-AP

FC experiment of HeLa using 10427-2-AP

WB analysis of HEK-293 using 10427-2-AP

IHC staining of human breast cancer using 10427-2-AP

IHC staining of human breast cancer using 10427-2-AP

IHC staining of human colon using 10427-2-AP

IF Staining of HepG2 using 10427-2-AP

Calnexin Polyclonal antibody

Calnexin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A virally encoded high-resolution screen of cytomegalovirus dependencies

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity

A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma

The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity