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验证数据展示

  • IF Staining of HepG2 using CL488-10427

    IF Staining of HepG2 using CL488-10427

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Calnexin antibody (CL488-10427) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-10427

    FC experiment of HeLa using CL488-10427

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Calnexin (CL488-10427) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Calnexin Polyclonal antibody

Calnexin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10427

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

CANX, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90, CNX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10427 targets Calnexin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Calnexin fusion protein Ag0697 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calnexin
别名 CANX, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90, CNX
计算分子量 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC003552
基因名称 Calnexin
Gene ID (NCBI) 821
RRIDAB_2918978
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27824
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Calnexin antibody CL488-10427Download protocol
FC protocol for CL Plus 488 Calnexin antibody CL488-10427Download protocol
Standard Protocols
Click here to view our Standard Protocols