验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells, UV treated Hela, RAW264.7 |
| Positive IP detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13423-1-AP targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP, ELISA |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep |
| 免疫原 | Caspase 8/P43/P18 fusion protein Ag4214 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 8, apoptosis-related cysteine peptidase |
| 别名 | CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4 |
| 计算分子量 | 538 aa, 62 kDa |
| 观测分子量 | 53-57 kDa, 32-45 kDa |
| GenBank蛋白编号 | BC028223 |
| 基因名称 | Caspase 8 |
| Gene ID (NCBI) | 841 |
| RRID | AB_2068463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 8/P43/P18 antibody 13423-1-AP | Download protocol |
| IHC protocol for Caspase 8/P43/P18 antibody 13423-1-AP | Download protocol |
| IF protocol for Caspase 8/P43/P18 antibody 13423-1-AP | Download protocol |
| IP protocol for Caspase 8/P43/P18 antibody 13423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Sci Adv HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling. | ||
Exp Mol Med PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer | ||
Nat Commun Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms. | ||
Aging Dis MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis. | ||
EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. |
