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CoraLite® Plus 488-conjugated Caspase 8/p43/p18 Polyclonal antibody
Caspase 8/p43/p18 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13423-1-AP
产品信息
CL488-13423 targets Caspase 8/p43/p18 in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | Caspase 8/p43/p18 fusion protein Ag4214 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | caspase 8, apoptosis-related cysteine peptidase |
Synonyms | Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5 |
Calculated Molecular Weight | 538 aa, 62 kDa |
Observed Molecular Weight | 53-57 kDa, 32-45 kDa |
GenBank Accession Number | BC028223 |
Gene Symbol | CASP8 |
Gene ID (NCBI) | 841 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14790 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 Caspase 8/p43/p18 antibody CL488-13423 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |