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验证数据展示

  • IF Staining of HepG2 using CL488-13423

    IF Staining of HepG2 using CL488-13423

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Caspase 8/P43/P18 antibody (CL488-13423) at dilution of 1:100, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-13423

    FC experiment of HepG2 using CL488-13423

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Caspase 8/P43/P18 Polyclonal Antibody (CL488-13423)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Caspase 8/P43/P18 Polyclonal antibody

Caspase 8/P43/P18 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13423

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13423-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13423 targets Caspase 8/P43/P18 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Caspase 8/P43/P18 fusion protein Ag4214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 8, apoptosis-related cysteine peptidase
别名 CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4
计算分子量 538 aa, 62 kDa
观测分子量 53-57 kDa, 32-45 kDa
GenBank蛋白编号BC028223
基因名称 Caspase 8
Gene ID (NCBI) 841
RRIDAB_3672564
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14790
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 8/P43/P18 antibody CL488-13423Download protocol
FC protocol for CL Plus 488 Caspase 8/P43/P18 antibody CL488-13423Download protocol
Standard Protocols
Click here to view our Standard Protocols