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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Caspase 8/p43/p18 Polyclonal antibody

Caspase 8/p43/p18 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13423

Print datasheet

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

验证数据展示

  • IF Staining of HepG2 using CL488-13423

    IF Staining of HepG2 using CL488-13423

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Caspase 8/p43/p18 antibody (CL488-13423) at dilution of 1:100, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-13423

    FC experiment of HepG2 using CL488-13423

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Caspase 8/P43/P18 Polyclonal Antibody (CL488-13423)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13423-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13423 targets Caspase 8/p43/p18 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Caspase 8/p43/p18 fusion protein Ag4214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 8, apoptosis-related cysteine peptidase
Synonyms CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5
Calculated Molecular Weight 538 aa, 62 kDa
Observed Molecular Weight 53-57 kDa, 32-45 kDa
GenBank Accession NumberBC028223
Gene Symbol Caspase 8
Gene ID (NCBI) 841
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14790
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 8/p43/p18 antibody CL488-13423Download protocol
FC protocol for CL Plus 488 Caspase 8/p43/p18 antibody CL488-13423Download protocol
Standard Protocols
Click here to view our Standard Protocols