验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-65051 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | Human |
| Immunogen | Human adherent peripheral blood cells 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lysosomal-associated membrane protein 1 |
| Synonyms | CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker |
| Calculated Molecular Weight | 45 kDa |
| GenBank Accession Number | BC006345 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2883468 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD107a / LAMP1 antibody CL594-65051 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |