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CoraLite® Plus 647 Anti-Human CD11c (3.9)

CD11c Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3.9

Cat no : CL647-65086

Print datasheet

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

验证数据展示

  • FC experiment of human peripheral blood monocytes using CL647-65086

    FC experiment of human peripheral blood monocytes using CL647-65086

    100 ul human peripheral blood were surface stained with 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD11c (CL647-65086, Clone:3.9) and then treated with red blood cell lysis buffer. Cells were not fixed.

  • FC experiment of human peripheral blood monocytes using CL647-65086

    FC experiment of human peripheral blood monocytes using CL647-65086

    100 ul human peripheral blood were surface stained with CoraLite® Plus 647-conjugated Mouse IgG1 isotype control and then treated with red blood cell lysis buffer. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood monocytes
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65086-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65086 targets CD11c in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Synonyms CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6
Calculated Molecular Weight 1169 aa, 129 kDa
GenBank Accession NumberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2883672
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP20702
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD11c antibody CL647-65086Download protocol
Standard Protocols
Click here to view our Standard Protocols