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CoraLite® Plus 488-conjugated CD13 Polyclonal antibody

CD13 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14553

Print datasheet

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

验证数据展示

  • IF Staining of mouse kidney using CL488-14553

    IF Staining of mouse kidney using CL488-14553

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CL488-14553 (CD13 antibody) at dilution of 1:50 .

  • IF Staining of mouse kidney using CL488-14553

    IF Staining of mouse kidney using CL488-14553

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CL488-14553 (CD13 antibody) at dilution of 1:50.

经过测试的应用

Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy CD13 Ready-To-Use IHC Kit. CD13 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14553-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14553 targets CD13 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen CD13 fusion protein Ag5976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanyl (membrane) aminopeptidase
Synonyms Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC058928
Gene Symbol CD13
Gene ID (NCBI) 290
ENSEMBL Gene IDENSG00000166825
RRIDAB_2883083
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD13 antibody CL488-14553Download protocol
Standard Protocols
Click here to view our Standard Protocols