验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65085-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65085 targets CD20 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Human |
| 免疫原 | Human tonsillar B cells 种属同源性预测 |
| 宿主/亚型 | Mouse / Mouse IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | membrane-spanning 4-domains, subfamily A, member 1 |
| 别名 | B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7 |
| 计算分子量 | 297 aa, 33 kDa |
| GenBank蛋白编号 | BC002807 |
| 基因名称 | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_2924086 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD20 antibody CL750-65085 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |