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PE Anti-Human CD20 (2H7)

CD20 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

2H7

Cat no : PE-65085

Print datasheet

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

验证数据展示

  • FC experiment of human PBMCs using PE-65085

    FC experiment of human PBMCs using PE-65085

    1X10^6 human PBMCs were surface co-stained with CL647 plus Anti-Human CD19 and 5 ul PE Anti-Human CD20 (PE-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using PE-65085

    FC experiment of human PBMCs using PE-65085

    1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD20 (PE-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65085 targets CD20 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human tonsillar B cells 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name membrane-spanning 4-domains, subfamily A, member 1
Synonyms B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC002807
Gene Symbol CD20
Gene ID (NCBI) 931
RRIDAB_2883879
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP11836
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

实验方案

Product Specific Protocols
FC protocol for PE CD20 antibody PE-65085Download protocol
Standard Protocols
Click here to view our Standard Protocols