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验证数据展示

  • WB analysis of Ramos using 66103-1-Ig (same clone as 66103-1-PBS)

    WB analysis of Ramos using 66103-1-Ig (same clone as 66103-1-PBS)

    Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

    WB analysis of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • IF Staining of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

    IF Staining of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • Cytometric bead array standard curve of MP50311-4

    Cytometric bead array standard curve of MP50311-4

    Cytometric bead array standard curve of MP50311-4, CD22 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66103-1-PBS. Detection antibody: 66103-6-PBS. Standard:Ag17986. Range: 0.781-100 ng/mL.

CD22 Monoclonal antibody, PBS Only (Capture)

CD22 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66103-1-PBS

产品说明书

CloneNo. 7A2F1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

产品信息

66103-1-PBS targets CD22 as part of a matched antibody pair:

MP50311-4: 66103-1-PBS capture and 66103-6-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD22 fusion protein Ag17986 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD22 molecule
别名 CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22
计算分子量 847 aa, 95 kDa
观测分子量 135 kDa
GenBank蛋白编号BC109306
基因名称 CD22
Gene ID (NCBI) 933
RRIDAB_2881502
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20273
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.