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CD23 Monoclonal antibody, PBS Only

CD23 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3H6B8

Cat No : 60208-1-PBS

Print datasheet

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

验证数据展示

  • WB analysis of DC2.4 using 60208-1-Ig (same clone as 60208-1-PBS)

    WB analysis of DC2.4 using 60208-1-Ig (same clone as 60208-1-PBS)

    DC2.4, Raji, Ramos and Daudi cells were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-1-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-1-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

产品信息

60208-1-PBS targets CD23 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen CD23 fusion protein Ag0425 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor
Calculated Molecular Weight 321 aa, 36 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC064417
Gene Symbol FCER2
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06734
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.