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CD23 Monoclonal antibody, PBS Only (Capture/Detector)

CD23 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H6B8

Cat no : 60208-1-PBS

Print datasheet

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

验证数据展示

  • WB analysis using 60208-1-Ig (same clone as 60208-1-PBS)

    WB analysis using 60208-1-Ig (same clone as 60208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-1-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-1-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50220-3

    Cytometric bead array standard curve of MP50220-3

    Cytometric bead array standard curve of MP50220-3, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-1-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP50220-4

    Cytometric bead array standard curve of MP50220-4

    Cytometric bead array standard curve of MP50220-4, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-1-PBS. Detection antibody: 60208-4-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

产品信息

60208-1-PBS targets CD23 as part of a matched antibody pair:

MP50220-3: 60208-2-PBS capture and 60208-1-PBS detection (validated in Cytometric bead array)

MP50220-4: 60208-1-PBS capture and 60208-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD23 fusion protein Ag0425 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
Calculated Molecular Weight 321 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06734
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.