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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66308-1-Ig (same clone as 66308-1-PBS)

    WB analysis using 66308-1-Ig (same clone as 66308-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 66308-1-Ig (same clone as 66308-1-PBS)

    FC experiment of human PBMCs using 66308-1-Ig (same clone as 66308-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 μg CD27 Monoclonal antibody (66308-1-Ig, Clone:1C1G3) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: T1F8D3F10), and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO.RGAM005). Cells were not fixed. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

CD27 Monoclonal antibody, PBS Only

CD27 Monoclonal Antibody for WB, IHC, IF-P, FC, ELISA
Cat No. 66308-1-PBS

产品说明书

CloneNo. 1C1G3

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF-P, FC, ELISA

别名

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66308-1-PBS targets CD27 in WB, IHC, IF-P, FC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, FC, ELISA Application Description
经测试反应性 human, pig
免疫原 CD27 fusion protein Ag24537 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD27 molecule
别名 T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen
计算分子量 29 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC012160
基因名称 CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_2881690
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26842
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.