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验证数据展示

  • IF Staining of human placenta using CL594-14486

    IF Staining of human placenta using CL594-14486

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD34 antibody (CL594-14486) at dilution of 1:200.

  • IF Staining of human placenta using CL594-14486

    IF Staining of human placenta using CL594-14486

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD34 antibody (CL594-14486) at dilution of 1:200.

CoraLite®594-conjugated CD34 Polyclonal antibody

CD34 Polyclonal Antibody for IF-P
Cat No. CL594-14486

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Synonyms

CD34, CD34 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue, human liver cancer tissue, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14486-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14486 targets CD34 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen CD34 fusion protein Ag5887 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD34 molecule
Synonyms CD34, CD34 molecule
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 105-120 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
RRIDAB_2923914
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28906
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.

实验方案

Product Specific Protocols
IF protocol for CL594 CD34 antibody CL594-14486Download protocol
Standard Protocols
Click here to view our Standard Protocols