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APC Anti-Human CD4 (OKT4)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

OKT4

Cat no : APC-65134

Print datasheet

Synonyms

CD4, CD4 molecule, CD4mut

验证数据展示

  • FC experiment of human peripheral blood lymphocytes using APC-65134

    FC experiment of human peripheral blood lymphocytes using APC-65134

    1X10^6 human peripheral blood lymphocytes were surface stained with0.125 ug APC-anti-human CD4 (APC-65134, clone OKT4) (red) or 0.125 ug APC-mouse IgG2b isotype control (blue). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using APC-65134

    FC experiment of human peripheral blood lymphocytes using APC-65134

    0.1 mL human whole blood cells were surface stained with 0.125 ug FITC-anti-human CD3 (FITC-65151, clone UCHT1) and 0.125 ug APC-anti-human CD4 (APC-65134, clone OKT4). Cells were then treated with red blood cell lysis buffer and were gated for lymphocytes for analysis of CD3 and CD4 staining. Cells were not fixed.

经过测试的应用

Positive FC detected inHuman peripheral blood lymphocytes

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65134 targets CD4 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human peripheral T cells 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD4 molecule
Synonyms CD4, CD4 molecule, CD4mut
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2883015
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01730
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for APC CD4 antibody APC-65134Download protocol
Standard Protocols
Click here to view our Standard Protocols