• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD40 (G28.5), PBS Only

CD40 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

G28.5

Cat no : 65103-1-PBS

Print datasheet

Synonyms

p50, G28.5, CD40L receptor, Bp50, B-cell surface antigen CD40

验证数据展示

  • FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50064-1

    Sandwich ELISA standard curve of MP50064-1

    Sandwich ELISA standard curve of MP50064-1, CD40 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66965-2-PBS. Detection antibody: 65103-1-PBS. Standard: Ag28040. Range: 78.1-5000 pg/mL.

产品信息

65103-1-PBS targets CD40 as part of a matched antibody pair:

MP50064-1: 66965-2-PBS capture and 65103-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD40 molecule, TNF receptor superfamily member 5
Synonyms p50, G28.5, CD40L receptor, Bp50, B-cell surface antigen CD40
Calculated Molecular Weight 277 aa, 31 kDa
GenBank Accession NumberBC012419
Gene Symbol CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_2881471
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).