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验证数据展示

  • FC experiment of human PBMCs using 65103-1-Ig

    FC experiment of human PBMCs using 65103-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65103-1-Ig

    FC experiment of human PBMCs using 65103-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for FC
Cat No. 65103-1-Ig

产品说明书

CloneNo. G28.5

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65103-1-Ig targets CD40 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD40 molecule, TNF receptor superfamily member 5
别名 B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5
计算分子量 277 aa, 31 kDa
GenBank蛋白编号BC012419
基因名称 CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_2881471
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

实验方案

Product Specific Protocols
FC protocol for CD40 antibody 65103-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols