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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL647-65103

    FC experiment of human peripheral blood lymphocytes using CL647-65103

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 647-conjugated Anti-Human CD40 (CL647-65103, Clone: G28.5) (red) or isotype control antibody (blue). Cells were not fixed.

CoraLite® Plus 647 Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for FC
Cat No. CL647-65103

产品说明书

CloneNo. G28.5

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65103-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65103 targets CD40 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD40 molecule, TNF receptor superfamily member 5
别名 B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5
计算分子量 277 aa, 31 kDa
GenBank蛋白编号BC012419
基因名称 CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_2883681
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD40 antibody CL647-65103Download protocol
Standard Protocols
Click here to view our Standard Protocols