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验证数据展示

  • IF Staining of MCF-7 using CL594-66180

    IF Staining of MCF-7 using CL594-66180

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CD71 Monoclonal antibody

CD71 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL594-66180

产品说明书

CloneNo. 3C11F11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, IF-P

别名

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells, human breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66180-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66180 targets CD71 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 CD71 fusion protein Ag21612 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transferrin receptor (p90, CD71)
别名 CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
计算分子量 85 kDa
观测分子量 90 kDa
GenBank蛋白编号BC001188
基因名称 CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
RRIDAB_2919961
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02786
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
IF protocol for CL594 CD71 antibody CL594-66180Download protocol
Standard Protocols
Click here to view our Standard Protocols