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验证数据展示

  • IF Staining of human colon cancer using CL594-66868

    IF Staining of human colon cancer using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green), CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta).

  • IF Staining of human tonsillitis using CL594-66868

    IF Staining of human tonsillitis using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66868 (CD8a antibody) at dilution of 1:50.

  • IF Staining of human tonsillitis using CL594-66868

    IF Staining of human tonsillitis using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:400. DAPI (blue).

CoraLite®594-conjugated CD8a Monoclonal antibody

CD8a Monoclonal Antibody for IF-P
Cat No. CL594-66868

产品说明书

CloneNo. 1G2B10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF-P

别名

CD8, 1G2B10, p32, MAL, Leu2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66868-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66868 targets CD8a in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 CD8a fusion protein Ag11111 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD8a molecule
别名 CD8, 1G2B10, p32, MAL, Leu2
计算分子量 235 aa, 26 kDa
GenBank蛋白编号BC025715
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2883630
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP01732
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

实验方案

Product Specific Protocols
IF protocol for CL594 CD8a antibody CL594-66868Download protocol
Standard Protocols
Click here to view our Standard Protocols