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验证数据展示

  • IF Staining of human colon cancer using CL594-66868

    IF Staining of human colon cancer using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green), CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta).

  • IF Staining of human tonsillitis using CL594-66868

    IF Staining of human tonsillitis using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66868 (CD8a antibody) at dilution of 1:50.

  • IF Staining of human tonsillitis using CL594-66868

    IF Staining of human tonsillitis using CL594-66868

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD8a antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:400. DAPI (blue).

CoraLite®594-conjugated CD8a Monoclonal antibody

CD8a Monoclonal Antibody for IF-P
Cat No. CL594-66868

产品说明书

CloneNo. 1G2B10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF-P

别名

CD8, 1G2B10, CD8a molecule, Leu2, MAL

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66868-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66868 targets CD8a in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag11111

Product name: Recombinant human CD8A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-183 aa of BC025715

Sequence: QFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGCYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD8a molecule
别名 CD8, 1G2B10, CD8a molecule, Leu2, MAL
计算分子量 235 aa, 26 kDa
GenBank蛋白编号BC025715
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2883630
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP01732
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

实验方案

Product Specific Protocols
IF protocol for CL594 CD8a antibody CL594-66868Download protocol
Standard Protocols
Click here to view our Standard Protocols
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