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Biotin-conjugated CD81 Monoclonal antibody

CD81 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

1G2C6

Cat no : Biotin-66866

Print datasheet

Synonyms

CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

验证数据展示

  • IHC staining of human liver using Biotin-66866

    IHC staining of human liver using Biotin-66866

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using Biotin-66866

    IHC staining of human liver using Biotin-66866

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66866 targets CD81 in IHC applications and shows reactivity with Human samples.

Tested Applications IHC Application Description
Tested Reactivity Human
Immunogen CD81 fusion protein Ag27298 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD81 molecule
Synonyms CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60033
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
IHC protocol for Biotin CD81 antibody Biotin-66866Download protocol
Standard Protocols
Click here to view our Standard Protocols