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验证数据展示

  • IHC staining of human liver using Biotin-66866

    IHC staining of human liver using Biotin-66866

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using Biotin-66866

    IHC staining of human liver using Biotin-66866

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Biotin-conjugated CD81 Monoclonal antibody

CD81 Monoclonal Antibody for IHC
Cat No. Biotin-66866

产品说明书

CloneNo. 1G2C6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IHC

别名

CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66866 targets CD81 in IHC applications and shows reactivity with Human samples.

经测试应用 IHC Application Description
经测试反应性 Human
免疫原 CD81 fusion protein Ag27298 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD81 molecule
别名 CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28
计算分子量 26 kDa
观测分子量 22 kDa
GenBank蛋白编号BC002978
基因名称 CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_3083812
偶联类型 Biotin
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60033
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
IHC protocol for Biotin CD81 antibody Biotin-66866Download protocol
Standard Protocols
Click here to view our Standard Protocols