验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-SF98095 targets CD9 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg1037 Product name: recombinant human CD9 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 112-195 aa of NM_001769.4 Sequence: SHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHI 种属同源性预测 |
| 宿主/类型 | Rabbit / scFv |
| 抗体类别 | Recombinant |
| 产品类型 | Single-chain variable fragment antibody (scFv) |
| 全称 | CD9 molecule |
| 别名 | 5H9, 5H9 antigen, BA2, BTCC 1, CD9 antigen |
| 计算分子量 | 25 kDa |
| GenBank蛋白编号 | NM_001769.4 |
| 基因名称 | CD9 |
| Gene ID (NCBI) | 928 |
| RRID | AB_3748003 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 来源 | Anti-Human CD9 scFv from clone 240830E6, consisting of VL-GGGGS linker-VH (N- to C-terminus), with a His tag at the C-terminus. |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 4% sucrose and 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD9 antibody CL488-SF98095 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
