验证数据展示
经过测试的应用
Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66912 targets CDC25C in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CDC25C fusion protein Ag10240 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | cell division cycle 25 homolog C (S. pombe) |
别名 | M-phase inducer phosphatase 3, M phase inducer phosphatase 3, EC:3.1.3.48, CDC25, 2B6B12 |
计算分子量 | 473 aa, 53 kDa |
观测分子量 | 46-53 kDa |
GenBank蛋白编号 | BC019089 |
基因名称 | CDC25C |
Gene ID (NCBI) | 995 |
RRID | AB_2923761 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P30307 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CDC25C antibody CL488-66912 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |