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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10122

Print datasheet

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

验证数据展示

  • FC experiment of MCF-7 using CL647-10122

    FC experiment of MCF-7 using CL647-10122

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CDK2 (CL647-10122) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using CL647-10122

    FC experiment of HeLa using CL647-10122

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated CDK2 Polyclonal antibody (CL647-10122)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10122-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10122 targets CDK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CDK2 fusion protein Ag0166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 2
Synonyms p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24941
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CDK2 antibody CL647-10122Download protocol
Standard Protocols
Click here to view our Standard Protocols