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验证数据展示

  • IF Staining of MCF-7 using CL555-11026

    IF Staining of MCF-7 using CL555-11026

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 CDK4 antibody (CL555-11026) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated CDK4 Polyclonal antibody

CDK4 Polyclonal Antibody for IF/ICC
Cat No. CL555-11026

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11026-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-11026 targets CDK4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CDK4 fusion protein Ag1505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 4
别名 CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC010153
基因名称 CDK4
Gene ID (NCBI) 1019
RRIDAB_2919596
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11802
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).

实验方案

Product Specific Protocols
IF protocol for CL555 CDK4 antibody CL555-11026Download protocol
Standard Protocols
Click here to view our Standard Protocols