验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, human brain tissue, human skin tissue, mouse brain tissue, NIH/3T3 cells, rat brain tissue, NCI-H1299 cells |
| Positive IHC detected in | human colon tissue, human cervical cancer tissue, human heart tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10960-1-AP targets Cofilin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, chicken |
| 免疫原 |
CatNo: Ag1399 Product name: Recombinant human Cofilin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC012318 Sequence: MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cofilin 1 (non-muscle) |
| 别名 | CFL1, 18 kDa phosphoprotein, Cofilin 1, Cofilin, non-muscle isoform, Cofilin1 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 18-22 kDa |
| GenBank蛋白编号 | BC012318 |
| 基因名称 | Cofilin |
| Gene ID (NCBI) | 1072 |
| RRID | AB_2291830 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23528 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cofilin antibody 10960-1-AP | Download protocol |
| IHC protocol for Cofilin antibody 10960-1-AP | Download protocol |
| WB protocol for Cofilin antibody 10960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.
| ||
Nat Commun TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis
| ||
Nat Commun N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization | ||
Nat Commun N-terminal α-amino SUMOylation promotes phosphorylation-independent cofilin-1 translocation to the mitochondrial matrix and induces apoptosis.
| ||
Cell Death Discov Protein arginine methyltransferase 1 stimulates basal cell proliferation and migration to maintain corneal epithelial homeostasis. | ||
Neural Regen Res No DCX-positive neurogenesis in the cerebral cortex of the adult primate. |
