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验证数据展示

  • IF Staining of NIH/3T3 using CL488-10960

    IF Staining of NIH/3T3 using CL488-10960

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 Cofilin antibody (CL488-10960) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cofilin Polyclonal antibody

Cofilin Polyclonal Antibody for IF/ICC
Cat No. CL488-10960

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10960-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10960 targets Cofilin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Cofilin fusion protein Ag1399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cofilin 1 (non-muscle)
Synonyms 18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18-22 kDa
GenBank Accession NumberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
RRIDAB_3672475
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23528
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cofilin antibody CL488-10960Download protocol
Standard Protocols
Click here to view our Standard Protocols