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验证数据展示

  • FC experiment of HEK-293 using CL488-25335

    FC experiment of HEK-293 using CL488-25335

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Chd3 Polyclonal Antibody (CL488-25335)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CHD3 Polyclonal antibody

CHD3 Polyclonal Antibody for FC (Intra)
Cat No. CL488-25335

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CHD3 Ready-To-Use IHC Kit. CHD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25335-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25335 targets CHD3 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromodomain helicase DNA binding protein 3
别名 hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3
计算分子量 227 kDa
观测分子量 240 kDa
GenBank蛋白编号NM_005852
基因名称 CHD3
Gene ID (NCBI) 1107
RRIDAB_3672791
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12873
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CHD3 antibody CL488-25335Download protocol
Standard Protocols
Click here to view our Standard Protocols