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CoraLite® Plus 488-conjugated CHD3 Polyclonal antibody

CHD3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25335

Print datasheet

Synonyms

hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3

验证数据展示

  • FC experiment of HEK-293 using CL488-25335

    FC experiment of HEK-293 using CL488-25335

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Chd3 Polyclonal Antibody (CL488-25335)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CHD3 Ready-To-Use IHC Kit. CHD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25335-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25335 targets CHD3 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromodomain helicase DNA binding protein 3
Synonyms hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3
Calculated Molecular Weight 227 kDa
Observed Molecular Weight 240 kDa
GenBank Accession NumberNM_005852
Gene Symbol CHD3
Gene ID (NCBI) 1107
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12873
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CHD3 antibody CL488-25335Download protocol
Standard Protocols
Click here to view our Standard Protocols