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CoraLite® Plus 488-conjugated CHEK2 Polyclonal antibody

CHEK2 Polyclonal Antibody for IF/ICC
Cat No. CL488-13954

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

CDS1, CHEK2, CHK2, HuCds1, LFS2, PP1425, RAD53

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CHEK2 Ready-To-Use IHC Kit. CHEK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13954-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13954 targets CHEK2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CHEK2 fusion protein Ag5065 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHK2 checkpoint homolog (S. pombe)
别名 CDS1, CHEK2, CHK2, HuCds1, LFS2, PP1425, RAD53
计算分子量 61 kDa
观测分子量 65 kDa
GenBank蛋白编号BC004207
基因名称 CHEK2
Gene ID (NCBI) 11200
RRIDAB_3083949
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO96017
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHEK2 antibody CL488-13954Download protocol
Standard Protocols
Click here to view our Standard Protocols
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