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验证数据展示

  • IF Staining of HepG2 using CL488-12036

    IF Staining of HepG2 using CL488-12036

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CHI3L1/YKL40 antibody (CL488-12036) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for IF/ICC
Cat No. CL488-12036

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12036-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12036 targets CHI3L1/YKL40 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 CHI3L1/YKL40 fusion protein Ag2710 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chitinase 3-like 1 (cartilage glycoprotein-39)
别名 CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39
计算分子量 383 aa, 43 kDa
观测分子量 40, 33 kDa
GenBank蛋白编号BC008568
基因名称 CHI3L1
Gene ID (NCBI) 1116
RRIDAB_3083912
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36222
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHI3L1/YKL40 antibody CL488-12036Download protocol
Standard Protocols
Click here to view our Standard Protocols