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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12036

Print datasheet

Synonyms

CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39

验证数据展示

  • IF Staining of HepG2 using CL488-12036

    IF Staining of HepG2 using CL488-12036

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CHI3L1/YKL40 antibody (CL488-12036) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12036-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12036 targets CHI3L1/YKL40 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen CHI3L1/YKL40 fusion protein Ag2710 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Synonyms CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 40, 33 kDa
GenBank Accession NumberBC008568
Gene Symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36222
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHI3L1/YKL40 antibody CL488-12036Download protocol
Standard Protocols
Click here to view our Standard Protocols