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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CLTA Polyclonal antibody

CLTA Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10852

Print datasheet

Synonyms

LCA, clathrin, light chain (Lca), Clathrin light chain A

验证数据展示

  • IF Staining of HepG2 using CL488-10852

    IF Staining of HepG2 using CL488-10852

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CLTA antibody (CL488-10852) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLTA Ready-To-Use IHC Kit. CLTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10852-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10852 targets CLTA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen CLTA fusion protein Ag1299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clathrin, light chain (Lca)
Synonyms LCA, clathrin, light chain (Lca), Clathrin light chain A
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC019287
Gene Symbol CLTA
Gene ID (NCBI) 1211
RRIDAB_2919004
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09496
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CLTA antibody CL488-10852Download protocol
Standard Protocols
Click here to view our Standard Protocols