验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HL-60 cells, Jurkat cells, mouse spleen tissue, rat brain tissue, rat spleen tissue, mouse thymus tissue |
| Positive IP detected in | mouse brain tissue, mouse spleen tissue |
| Positive IHC detected in | rat spleen tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, mouse colon tissue |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
17760-1-AP targets CORO1A in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | CORO1A fusion protein Ag12139 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coronin, actin binding protein, 1A |
| 别名 | Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A |
| 计算分子量 | 461 aa, 51 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC110374 |
| 基因名称 | CORO1A |
| Gene ID (NCBI) | 11151 |
| RRID | AB_2082070 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31146 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CORO1A antibody 17760-1-AP | Download protocol |
| IHC protocol for CORO1A antibody 17760-1-AP | Download protocol |
| IF protocol for CORO1A antibody 17760-1-AP | Download protocol |
| IP protocol for CORO1A antibody 17760-1-AP | Download protocol |
| FC protocol for CORO1A antibody 17760-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash. | ||
Front Med (Lausanne) MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene. | ||
PLoS One Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia. | ||
Theranostics TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy |
