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CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 17760-1-AP

Print datasheet

Synonyms

Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A

验证数据展示

  • WB analysis of rat brain using 17760-1-AP

    WB analysis of rat brain using 17760-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 17760-1-AP

    IP experiment of mouse brain using 17760-1-AP

    IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:3000) with mouse brain tissue lysate 1840 ug.

  • IP experiment of mouse spleen using 17760-1-AP

    IP experiment of mouse spleen using 17760-1-AP

    IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.

  • FC experiment of Jurkat using 17760-1-AP

    FC experiment of Jurkat using 17760-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CORO1A (17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of mouse spleen using 17760-1-AP

    WB analysis of mouse spleen using 17760-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 17760-1-AP

    IHC staining of mouse spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CORO1A antibody included
  • IHC staining of mouse spleen using 17760-1-AP

    IHC staining of mouse spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CORO1A antibody included
  • IHC staining of rat spleen using 17760-1-AP

    IHC staining of rat spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CORO1A antibody included
  • IHC staining of rat spleen using 17760-1-AP

    IHC staining of rat spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CORO1A antibody included
  • IF Staining of mouse spleen using 17760-1-AP

    IF Staining of mouse spleen using 17760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse colon using 17760-1-AP

    IF Staining of mouse colon using 17760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse spleen using 17760-1-AP

    IF Staining of mouse spleen using 17760-1-AP

    Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse spleen tissue, HL-60 cells, Jurkat cells, rat brain tissue, rat spleen tissue
Positive IP detected inmouse brain tissue, mouse spleen tissue
Positive IHC detected inrat spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse colon tissue
Positive IF-Fro detected inmouse spleen tissue
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

17760-1-AP targets CORO1A in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen CORO1A fusion protein Ag12139 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coronin, actin binding protein, 1A
Synonyms Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
RRIDAB_2082070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31146
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

实验方案

Product Specific Protocols
WB protocol for CORO1A antibody 17760-1-APDownload protocol
IHC protocol for CORO1A antibody 17760-1-APDownload protocol
IF protocol for CORO1A antibody 17760-1-APDownload protocol
IP protocol for CORO1A antibody 17760-1-APDownload protocol
FC protocol for CORO1A antibody 17760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Br J Pharmacol

PMID: 27128203

Authors - Xianrong Zhang
View Article
humanWB

Int Immunopharmacol

Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.

Authors - Fuli Min
View Article
humanWB

Front Med (Lausanne)

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Authors - Hui Song
View Article
ratWB

PLoS One

Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.

Authors - Feng Guo
View Article