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CoraLite® Plus 488-conjugated CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17760

Print datasheet

Synonyms

Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A

验证数据展示

  • FC experiment of Jurkat using CL488-17760

    FC experiment of Jurkat using CL488-17760

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CORO1A (CL488-17760) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human colon using CL488-17760

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of human colon using CL488-17760

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-17760

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-17760

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman colon tissue, mouse spleen tissue
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17760-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17760 targets CORO1A in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CORO1A fusion protein Ag12139 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coronin, actin binding protein, 1A
Synonyms Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31146
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CORO1A antibody CL488-17760Download protocol
Standard Protocols
Click here to view our Standard Protocols