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MTCO2 Polyclonal antibody

MTCO2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

119

Cat no : 55070-1-AP

Print datasheet

Synonyms

COX2, mtcox2, MT CO2, COXII, COX 2

验证数据展示

WB analysis using 55070-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of 143B.TK.P0 using 55070-1-AP
WB result of MTCO2 antibody (MTCO2; 1:2000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.
WB analysis of various samples using 55070-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
IP experiment of HeLa using 55070-1-AP
IP result of anti-MTCO2 (IP:55070-1-AP, 4ug; Detection:55070-1-AP 1:1000) with HeLa cells lysate 1880 ug.
FC experiment of A431 using 55070-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MTCO2 (55070-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of human colon cancer using 55070-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MTCO2/COX2 antibody included
IF Staining of HepG2 using 55070-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (55070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 55070-1-AP

Size

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经过测试的应用

Positive WB detected in	A431 cells, 143B cells, various samples, HeLa cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	A431 cells

Planning an IHC experiment? We recommend our IHCeasy MTCO2/COX2 Ready-To-Use IHC Kit. MTCO2/COX2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 108 publications below
IHC	See 7 publications below
IF	See 5 publications below
IP	See 1 publications below
CoIP	See 1 publications below

产品信息

55070-1-AP targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human
Cited Reactivity	human, mouse, rat, monkey, zebrafish, hamster
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	cytochrome c oxidase II
Synonyms	COX2, mtcox2, MT CO2, COXII, COX 2
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	23-26 kDa
GenBank Accession Number	YP_003024029
Gene Symbol	MTCO2
Gene ID (NCBI)	4513
RRID	AB_10859832
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00403
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.

实验方案

Product Specific Protocols
WB protocol for MTCO2 antibody 55070-1-AP	Download protocol
IHC protocol for MTCO2 antibody 55070-1-AP	Download protocol
IF protocol for MTCO2 antibody 55070-1-AP	Download protocol
IP protocol for MTCO2 antibody 55070-1-AP	Download protocol
FC protocol for MTCO2 antibody 55070-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Cell Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. Authors - Paul K Ziegler View Article
human	WB	Cell Metab Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits. Authors - Ruchi Masand View Article
human	WB	Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. Authors - Yi-Ping Wang View Article
human	WB	Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Authors - Fangzhou Lou View Article
mouse	WB	Cell Res Active RNA interference in mitochondria. Authors - Kuanxing Gao View Article
human	WB	Mol Cell Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts. Authors - Xiaoyu Li View Article

MTCO2 Polyclonal antibody

MTCO2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 55070-1-AP

WB analysis of 143B.TK.P0 using 55070-1-AP

WB analysis of various samples using 55070-1-AP

IP experiment of HeLa using 55070-1-AP

FC experiment of A431 using 55070-1-AP

IHC staining of human colon cancer using 55070-1-AP

IF Staining of HepG2 using 55070-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Active RNA interference in mitochondria.

Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.

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