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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated MTCO2 Polyclonal antibody

MTCO2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-55070

Print datasheet

Synonyms

COX2, mtcox2, MT CO2, COXII, COX 2

验证数据展示

  • IF Staining of HepG2 using CL647-55070

    IF Staining of HepG2 using CL647-55070

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 MTCO2 antibody (CL647-55070) at dilution of 1:200.

  • FC experiment of HeLa using CL647-55070

    FC experiment of HeLa using CL647-55070

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated MTCO2 Polyclonal antibody (CL647-55070)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MTCO2/COX2 Ready-To-Use IHC Kit. MTCO2/COX2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55070-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-55070 targets MTCO2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase II
Synonyms COX2, mtcox2, MT CO2, COXII, COX 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberYP_003024029
Gene Symbol MTCO2
Gene ID (NCBI) 4513
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX2, also named as COII, COXII and MTCO2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to COX2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 MTCO2 antibody CL647-55070Download protocol
FC protocol for CL Plus 647 MTCO2 antibody CL647-55070Download protocol
Standard Protocols
Click here to view our Standard Protocols