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Calnexin Recombinant antibody

Calnexin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3K18

Cat no : 81938-1-RR

Print datasheet

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

验证数据展示

  • WB analysis using 81938-1-RR

    WB analysis using 81938-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81938-1-RR (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 81938-1-RR

    IHC staining of human cervical cancer using 81938-1-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calnexin antibody included
  • IHC staining of human cervical cancer using 81938-1-RR

    IHC staining of human cervical cancer using 81938-1-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calnexin antibody included
  • IF Staining of HepG2 using 81938-1-RR

    IF Staining of HepG2 using 81938-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81938-1-RR

    Affinity and Kinetic Characterization of 81938-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81938-1-RR against Human Calnexin were performed. The affinity constant is 1.76 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

81938-1-RR targets Calnexin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen Calnexin fusion protein Ag0697 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calnexin
Synonyms calnexin, CANX, CNX, FLJ26570, IP90, P90
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC003552
Gene Symbol Calnexin
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27824
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

实验方案

Product Specific Protocols
WB protocol for Calnexin antibody 81938-1-RRDownload protocol
IHC protocol for Calnexin antibody 81938-1-RRDownload protocol
IF protocol for Calnexin antibody 81938-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Extracell Biol

Bone marrow-fibroblast progenitor cell-derived small extracellular vesicles promote cardiac fibrosis via miR-21-5p and integrin subunit αV signalling

Authors - Prabhat Ranjan
View Article