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Calnexin Recombinant antibody, PBS Only

Calnexin Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K18

Cat No : 81938-1-PBS

Print datasheet

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

验证数据展示

  • WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

    WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81938-1-RR (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calnexin antibody included
  • IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calnexin antibody included
  • IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

    IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 81938-1-RR

    Affinity experiments of 81938-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81938-1-RR against Human Calnexin were performed. The affinity constant is 1.76 nM.

Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

产品信息

81938-1-PBS targets Calnexin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Calnexin fusion protein Ag0697 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calnexin
Synonyms calnexin, CANX, CNX, FLJ26570, IP90, P90
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC003552
Gene Symbol CANX
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27824
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.