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验证数据展示

  • WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

    WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81938-1-RR (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calnexin antibody included
  • IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calnexin antibody included
  • IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

    IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81938-1-RR

    Affinity and Kinetic Characterization of 81938-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81938-1-RR against Human Calnexin were performed. The affinity constant is 1.76 nM.

Calnexin Recombinant antibody, PBS Only

Calnexin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81938-1-PBS

产品说明书

CloneNo. 3K18

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CANX, 3K18, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

产品信息

81938-1-PBS targets Calnexin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Calnexin fusion protein Ag0697 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calnexin
别名 CANX, 3K18, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90
计算分子量 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC003552
基因名称 Calnexin
Gene ID (NCBI) 821
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP27824
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.