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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Calpain 1 Polyclonal antibody

Calpain 1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10538

Print datasheet

Synonyms

Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

验证数据展示

  • FC experiment of HeLa using CL647-10538

    FC experiment of HeLa using CL647-10538

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Calpain 1 (CL647-10538) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10538-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10538 targets Calpain 1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Calpain 1 fusion protein Ag0810 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain 1, (mu/I) large subunit
Synonyms Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL
Calculated Molecular Weight 704 aa, 82 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC008751
Gene Symbol CAPN1
Gene ID (NCBI) 823
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07384
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Calpain 1 antibody CL647-10538Download protocol
Standard Protocols
Click here to view our Standard Protocols