验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue |
| Positive IHC detected in | rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 7 publications below |
产品信息
68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | Cardiac Troponin T fusion protein Ag7716 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | troponin T type 2 (cardiac) |
| 别名 | TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC002653 |
| 基因名称 | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3085051 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P45379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| IHC protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| IF protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation | ||
Biochem Pharmacol Trimetazidine attenuates Ischemia/Reperfusion-Induced myocardial ferroptosis by modulating the Sirt3/Nrf2-GSH system and reducing Oxidative/Nitrative stress | ||
J Inflamm Res Cardamonin Attenuates Myocardial Ischemia/Reperfusion-Induced Ferroptosis Through Promoting STAT3 Signaling | ||
Clin Exp Hypertens Exploration and validation of signature genes and immune associations in septic cardiomyopathy | ||
J Transl Int Med WWP2 protects against sepsis-induced cardiac injury through inhibiting cardiomyocyte ferroptosis | ||
Toxicol Lett Roles of AhR/CYP1s Signal Pathway Mediated ROS Production in Uremic Cardiomyopathy |