Cardiac Troponin T Monoclonal antibody

Cardiac Troponin T Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, rabbit, chicken and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4E2D8

Cat no : 68300-1-Ig

Print datasheet

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8



经过测试的应用

Positive WB detected inrat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 6 publications below

产品信息

68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse, rat, pig, rabbit, chicken samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity mouse, rat, pig, rabbit, chicken
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen Cardiac Troponin T fusion protein Ag7716 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name troponin T type 2 (cardiac)
Synonyms TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8
Calculated Molecular Weight 36 kDa
Observed Molecular Weight34-40 kDa
GenBank Accession NumberBC002653
Gene Symbol Cardiac Troponin T
Gene ID (NCBI) 7139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP45379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

实验方案

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
IHC protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
IF protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rabbitIF

Adv Mater

DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation

Authors - Hangwei Chen
ratIF

Biochem Pharmacol

Trimetazidine attenuates Ischemia/Reperfusion-Induced myocardial ferroptosis by modulating the Sirt3/Nrf2-GSH system and reducing Oxidative/Nitrative stress

Authors - Mingyue Tan
mouseIF

Clin Exp Hypertens

Exploration and validation of signature genes and immune associations in septic cardiomyopathy

Authors - Zhengbo Zhao
ratIF

J Transl Int Med

WWP2 protects against sepsis-induced cardiac injury through inhibiting cardiomyocyte ferroptosis

Authors - Zhi Li
ratIF

Toxicol Lett

Roles of AhR/CYP1s Signal Pathway Mediated ROS Production in Uremic Cardiomyopathy

Authors - Wei Lu
humanIF

Eur J Pharmacol

Forskolin is an effective therapeutic small molecule for the treatment of hypertrophic cardiomyopathy through ADCY6/cAMP/PKA pathway

Authors - Wenyan Wang