验证数据展示
经过测试的应用
| Positive IF/ICC detected in | H9C2 cells |
For other applications, we recommend the unconjugated version of this antibody, 68300-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68300 targets Cardiac Troponin T in IF/ICC applications and shows reactivity with mouse, rat, rabbit, pig, chicken samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | mouse, rat, rabbit, pig, chicken |
| Immunogen | Cardiac Troponin T fusion protein Ag7716 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | troponin T type 2 (cardiac) |
| Synonyms | Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC002653 |
| Gene Symbol | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3084469 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P45379 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Cardiac Troponin T antibody CL488-68300 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |