验证数据展示
经过测试的应用
| Positive IF/ICC detected in | H9C2 cells |
For other applications, we recommend the unconjugated version of this antibody, 68300-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68300 targets Cardiac Troponin T in IF/ICC applications and shows reactivity with mouse, rat, rabbit, pig, chicken samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | mouse, rat, rabbit, pig, chicken |
| 免疫原 | Cardiac Troponin T fusion protein Ag7716 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | troponin T type 2 (cardiac) |
| 别名 | Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC002653 |
| 基因名称 | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3084469 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P45379 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Cardiac Troponin T antibody CL488-68300 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |