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CoraLite® Plus 488-conjugated Caveolin-1 Monoclonal antibody
Caveolin-1 Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF-P, FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6C2B2
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue, human skin cancer tissue |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Caveolin-1 Ready-To-Use IHC Kit. Caveolin-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66067-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66067 targets Caveolin-1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | Caveolin-1 fusion protein Ag8049 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | caveolin 1, caveolae protein, 22kDa |
| Synonyms | Caveolin 1, CAV1, CGL3, CAV, BSCL3 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 20-25 kDa |
| GenBank Accession Number | BC006432 |
| Gene Symbol | CAV1 |
| Gene ID (NCBI) | 857 |
| RRID | AB_2883249 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q03135 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Caveolin-1 antibody CL488-66067 | Download protocol |
| FC protocol for CL Plus 488 Caveolin-1 antibody CL488-66067 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
