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  • Featured Product
  • KD/KO Validated

Cyclin D1 Monoclonal antibody, PBS Only

Cyclin D1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G3G5

Cat no : 60186-1-PBS

Print datasheet

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

验证数据展示

  • WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60186-1-Ig (same clone as 60186-1-PBS)

    WB analysis of HeLa using 60186-1-Ig (same clone as 60186-1-PBS)

    WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 60186-1-Ig (same clone as 60186-1-PBS)

    WB analysis of RAW 264.7 using 60186-1-Ig (same clone as 60186-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

产品信息

60186-1-PBS targets Cyclin D1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cyclin D1 fusion protein Ag0689 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cyclin D1
Synonyms B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000076
Gene Symbol Cyclin D1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24385
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.