Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Cyclin D1 Monoclonal antibody (60186-1-Ig, Clone:2G3G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.