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验证数据展示

  • FC experiment of HeLa using CR-60186

    FC experiment of HeLa using CR-60186

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human Cyclin D1 (CR-60186, Clone:2G3G5) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using CR-60186

    WB analysis using CR-60186

    Various lysates were subjected to SDS PAGE followed by western blot with CR-60186 (Cyclin D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

Cardinal Red™-conjugated Cyclin D1 Monoclonal antibody

Cyclin D1 Monoclonal Antibody for WB, FC (Intra)
Cat No. CR-60186

Print datasheet

CloneNo. 2G3G5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Cardinal Red™
Size: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, NIH/3T3 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-60186 targets Cyclin D1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Cyclin D1 fusion protein Ag0689 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cyclin D1
Synonyms CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000076
Gene Symbol Cyclin D1
Gene ID (NCBI) 595
RRIDAB_2934343
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24385
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

实验方案

Product Specific Protocols
WB protocol for Cardinal Red™ Cyclin D1 antibody CR-60186Download protocol
FC protocol for Cardinal Red™ Cyclin D1 antibody CR-60186Download protocol
Standard Protocols
Click here to view our Standard Protocols