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Cardinal Red™-conjugated Cyclin D1 Monoclonal antibody

Cyclin D1 Monoclonal Antibody for WB, FC (Intra)
Cat No. CR-60186

产品说明书

CloneNo. 2G3G5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, NIH/3T3 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-60186 targets Cyclin D1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Cyclin D1 fusion protein Ag0689 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin D1
别名 CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein
计算分子量 295 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC000076
基因名称 Cyclin D1
Gene ID (NCBI) 595
RRIDAB_2934343
偶联类型 Cardinal Red™ Fluorescent Dye
最大激发/发射波长592 nm / 611 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24385
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

实验方案

Product Specific Protocols
WB protocol for Cardinal Red™ Cyclin D1 antibody CR-60186Download protocol
FC protocol for Cardinal Red™ Cyclin D1 antibody CR-60186Download protocol
Standard Protocols
Click here to view our Standard Protocols
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