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验证数据展示

  • IF Staining of HeLa using CL647-17516

    IF Staining of HeLa using CL647-17516

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 Cytokeratin 17-Specific antibody (CL647-17516) at dilution of 1:200.

  • FC experiment of HeLa using CL647-17516

    FC experiment of HeLa using CL647-17516

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cytokeratin 17-Specific (CL647-17516) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cytokeratin 17-Specific Polyclonal antibody

Cytokeratin 17-Specific Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-17516

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17516-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-17516 targets Cytokeratin 17-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 17
Synonyms 39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_000422
Gene Symbol Cytokeratin 17
Gene ID (NCBI) 3872
RRIDAB_2934927
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04695
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Cytokeratin 17-Specific antibody CL647-17516Download protocol
FC protocol for CL Plus 647 Cytokeratin 17-Specific antibody CL647-17516Download protocol
Standard Protocols
Click here to view our Standard Protocols