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Cytokeratin 20 Monoclonal antibody, PBS Only

Cytokeratin 20 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

4D10A4

Cat No : 60183-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT

验证数据展示

  • WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

    IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

产品信息

60183-1-PBS targets Cytokeratin 20 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen Cytokeratin 20 fusion protein Ag11336 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 20
Synonyms Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC031559
Gene Symbol KRT20
Gene ID (NCBI) 54474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.