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Cytokeratin 20 Monoclonal antibody, PBS Only

Cytokeratin 20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D10A4

Cat no : 60183-1-PBS

Print datasheet

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

验证数据展示

  • WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

    IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    IF Staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:400 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Collagen Type III (N-terminal) antibody (22734-1-AP, green), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

产品信息

60183-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Cytokeratin 20 fusion protein Ag11336 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name keratin 20
Synonyms KRT20, keratin 20, K20, Cytokeratin-20, CK-20
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC031559
Gene Symbol Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_10858399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.